▸ Current small molecule covalent inhibitors use Michael addition reaction of sulfhydryl (-SH) with Michael acceptors to target Cysteine(Cysteine)

▸ The terminal amino group (-NH2) of Lysine is another residue that can form covalent bonds, hydrogen bonds or other strong interactions with small molecules

▸ It is difficult for catalytical lysine to acquire adoptive mutation

▸ Small molecule inhibitors that target catalytic lysine are expected to solve the problem of TKI acquired resistance

▸ Design warhead targeting lysine based on the target protein’s structure

▸ Time-dependent in vivo and in vitro screening methods (lysine's covalent reaction or strong interaction leading to slow-off effects)

▸ Target undruggable targets

▸ Protein degradation leads to different biological effects

▸ Avoid On-target toxicity

▸ The function of molecular glue ternary complex structure (inhibition or degradation)

- Wigen monoclonal antibody and linker (Linker)

▸ New targets

▸ New Linker technologies

- Wigen's Payload

▸ Small molecule toxins, immunomodulatory molecules and PROTAC, etc.

▸ Higher Potency

▸ Ideal Physical and Chemical properities

- Create new biology

▸ ADCC effect of antibodies

▸ Pharmacological effects of antibodies

▸ Synergistic effect of small molecule payloads and antibodies